AIM: To determine the unknown concentration by fluorimetry.
Requirements:
Instrument: - Photofluorometer, Electronic balance (LCGC).
Apparatus: -Conical flask, beaker, stirrer, funnel, R.B.F., Pipette.
Chemicals: - Sulphuric Acid (0.1M).
Sample: - Marketed Quinine Sulphate tablet.
References:
Principle:
Fluorescence is the emission of light from a molecule, which return to its normal ground state from lowest vibrational level that is V0 of the excited singlet state. Excitation of molecule is achieved by exposing them to a light source of specified wavelength. Intensity of fluorescence is directly proportional to the concentration of fluorescent species. The relationship is observed for dilute solution only.
Procedure:-
Formula:
U-B=Conc. Of unknown
S-B=Conc. Of standard
Conc. Of unknown=U-B X conc. Of std S-B
Preparation of 0.1M Sulphuric acid: - Add 5.4ml of conc. Sulphuric acid slowly in 1000ml volumetric flash containing water and dilute up to mark.
Result: Concentration of unknown sample by calculation using formula was found to be______________ µg/100ml.
Remark:
Practical Performance
(2)
Conduct in Lab
(2)
Journal
(2)
Observations and Results
(2)
Viva-Voce
(2)
Total
(10)
Signature of Faculty In charge
Observation:
Concentration(ug/ml)
Absorbanceat nm
2
4
6
8
10
Slope
Intercept
Out R2
EXPERIMENT NO: 12
